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This reaction step was followed by the first wash step (500l P1, 400rpm, 5min), conjugation (100l of 1 1:5,000 diluted Protein A/G-HRP or anti-human-IgG-HRP (Sigma), 300rpm, 30min), and the second wash step (as above)

This reaction step was followed by the first wash step (500l P1, 400rpm, 5min), conjugation (100l of 1 1:5,000 diluted…

This reaction step was followed by the first wash step (500l P1, 400rpm, 5min), conjugation (100l of 1 1:5,000 diluted Protein A/G-HRP or anti-human-IgG-HRP (Sigma), 300rpm, 30min), and the second wash step (as above) Read More