Multiparameter flow cytometric analysis was performed for CD161, CD25, FoxP3, CD127, and Helios in freshly isolated cells or after co-culture for 7 days

Multiparameter flow cytometric analysis was performed for CD161, CD25, FoxP3, CD127, and Helios in freshly isolated cells or after co-culture…

FACS analysis was carried out using Fpro0165 IgG, and a mouse and a mouse anti-human FPR2 antibody (unpublished in-house reagent) anti-human CXCR3 antibody (R&D Systems, MAB160) with detection using either a PE-labeled anti-human IgG (Sigma P8047) or a PE-labeled anti mouse IgG1(Jackson ImmunoResearch,115-116-146) as appropriate

FACS analysis was carried out using Fpro0165 IgG, and a mouse and a mouse anti-human FPR2 antibody (unpublished in-house reagent)…