Therefore, FcRIIIa AC-MS will be an enormously handy tool in studying and tuning ADCC activation of (therapeutic) antibodies. glycosylation of…
The rescued cells were cultured in Gibco high glucose Dulbeccos Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin
The rescued cells were cultured in Gibco high glucose Dulbeccos Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS)…
The cells were co-incubated at 37C in 5% CO2for 4 days
The cells were co-incubated at 37C in 5% CO2for 4 days. we found that TCS increased the percentage of effector…
Supplementary horseradish peroxidase (HRP)-conjugated antibodies (1:2000; Invitrogen, Carlsbad, USA; “type”:”entrez-nucleotide”,”attrs”:”text”:”G21040″,”term_id”:”1341366″,”term_text”:”G21040″G21040, “type”:”entrez-nucleotide”,”attrs”:”text”:”G21234″,”term_id”:”1341560″,”term_text”:”G21234″G21234) were utilized based on the producers guidelines, and binding was discovered using a sophisticated chemiluminescence (ECL) recognition package (Amersham Pharmacia Biotek, Amersham, UK)
Supplementary horseradish peroxidase (HRP)-conjugated antibodies (1:2000; Invitrogen, Carlsbad, USA; “type”:”entrez-nucleotide”,”attrs”:”text”:”G21040″,”term_id”:”1341366″,”term_text”:”G21040″G21040, “type”:”entrez-nucleotide”,”attrs”:”text”:”G21234″,”term_id”:”1341560″,”term_text”:”G21234″G21234) were utilized based on the producers guidelines, and binding…