The rescued cells were cultured in Gibco high glucose Dulbeccos Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS)…
The cells were co-incubated at 37C in 5% CO2for 4 days
The cells were co-incubated at 37C in 5% CO2for 4 days. we found that TCS increased the percentage of effector…
Supplementary horseradish peroxidase (HRP)-conjugated antibodies (1:2000; Invitrogen, Carlsbad, USA; “type”:”entrez-nucleotide”,”attrs”:”text”:”G21040″,”term_id”:”1341366″,”term_text”:”G21040″G21040, “type”:”entrez-nucleotide”,”attrs”:”text”:”G21234″,”term_id”:”1341560″,”term_text”:”G21234″G21234) were utilized based on the producers guidelines, and binding was discovered using a sophisticated chemiluminescence (ECL) recognition package (Amersham Pharmacia Biotek, Amersham, UK)
Supplementary horseradish peroxidase (HRP)-conjugated antibodies (1:2000; Invitrogen, Carlsbad, USA; “type”:”entrez-nucleotide”,”attrs”:”text”:”G21040″,”term_id”:”1341366″,”term_text”:”G21040″G21040, “type”:”entrez-nucleotide”,”attrs”:”text”:”G21234″,”term_id”:”1341560″,”term_text”:”G21234″G21234) were utilized based on the producers guidelines, and binding…